GAI and GRS contain nuclear localization signals, a region of homology to a putative transcription factor, and motifs characteristic of transcriptional coactivators. However, interpretation of epistasis relationships in terms of the ordering of gene functions in pathways is not always a simple matter Avery and Wasserman First, we compare the effects of spy-7 and gar alone and in combination on the growth of and PAC resistance of plants containing gai. Received Aug 6; Accepted Sep Isolation and expression of three gibberellin oxidase cDNA clones from Arabidopsis. GA-deficient Arabidopsis mutants display characteristic phenotypes, including dark green leaves and a dwarf growth habit attributable to reduced stem elongation Koornneef and van der Veen ; Talon et al. Discussion The cloning of GAI through insertional mutagenesis of the gai allele demonstrates that gai is a gain-of-function, rather than a dominant loss-of-function mutation. RNA gel-blot analysis was used to visualize C oxidase transcripts in gai and in the gai-suppressor mutants Fig. Alternatively, the segment deleted in gai may have some other function, and the gai mutant protein may be locked into a repressive conformation for reasons other than the loss of a segment that interacts with the GA signal.
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